| Issue |
Acta Acust.
Volume 6, 2022
|
|
|---|---|---|
| Article Number | 11 | |
| Number of page(s) | 12 | |
| Section | Ultrasonics | |
| DOI | https://doi.org/10.1051/aacus/2022007 | |
| Published online | 29 March 2022 | |
Short Communication
Anti-reflection cover to control acoustic intensity in in vitro low-intensity ultrasound stimulation of cells
1
Aix Marseille Univ, CNRS, Centrale Marseille, IRPHE UMR 7342, 13013 Marseille, France
2
Aix Marseille Univ, CNRS, ISM UMR 7287, 13009 Marseille, France
3
Aix Marseille Univ, CNRS, Centrale Marseille, LMA UMR 7031, 13013 Marseille, France
4
Aix Marseille Univ, CNRS/IN2P3, CPPM UMR 7346, 13009 Marseille, France
5
Université Côte d’Azur, CNRS, Inserm, iBV, 06108 Nice, France
* Corresponding author: lasaygues@lma.cnrs-mrs.fr
Received:
12
November
2021
Accepted:
21
February
2022
Low-intensity ultrasound stimulation is a technique used in therapeutic ultrasound for bone regeneration. However, the underlying mechanisms are still poorly understood. In vitro studies on cell cultures are implemented to understand the processes involved. To analyze the effects of ultrasonic waves on cells, the control of the delivered acoustic intensity is essential. However, depending on the insonification protocol chosen, multiple reflections and standing waves that form inside the culture medium strongly hinder the estimates. In this work, we propose the development and the experimental validation of an anti-reflection cover. We demonstrate that this custom-designed device is effective in avoiding multiple reflections and makes it possible to artificially replace the layer of culture medium with a large amount of water. Finally, an analytical study of the acoustic intensity delivered to the cells is proposed.
Key words: Ultrasound stimulation / Cell-free culture medium / Acoustic intensity / Anti-reflection cover
© 2022 EDP Sciences
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